Hematoxylin Eosin (HE) 染色 (松岡俊樹)

 

脱パラフィン
  ↓  Xylen  for 5min
  ↓  Xylen  for 5min
  ↓  Xylen  for 5min
  ↓  Xylen  for 5min

親水
  ↓  100% Ethanol  for 3sec
  ↓  100% Ethanol  for 3sec
  ↓  95% Ethanol for 3sec
  ↓  90% Ethanol for 3sec
  ↓  80% Ethanol for 3sec
  ↓  流水洗浄(水道水) for 2~3min  ←水をはじかなくなるまで行なう 
 (↓  PBS  for 5min ×3 )
 
HE染色 (Mayer's Hematoxylin)
  ↓  Mayer's Hematoxylin液 for 15~20min 
↓  流水洗浄  ←洗浄水に色素の色がなくなるまで行なう
↓  1% HCl・70% Ethanol for 1sec
↓  流水洗浄  
(↓ 飽和炭酸リチウム for 1sec)  ←色出し  
 (↓ 流水洗浄(DW) for 10min) 
 ↓  蒸留水に軽く通す  ←2~3回up-down
↓ eosin液  ←2~3回up-down
  ↓ 余分の液を流し去る程度の洗浄(DW)←2~3回up-down 
  
脱水
↓  70% Ethanol for 3sec
↓  80% Ethanol for 3sec
↓  90% Ethanol for 3sec
↓  95% Ethanol for 3sec
↓  100% Ethanol for 5sec
↓  100% Ethanol for 5sec
透徹
↓  Xylen  for 5min
↓  Xylen  for 5min
↓  Xylen  for 5min
↓  Xylen  for 5min
封入
↓  EUKITT で封入