Hematoxylin Eosin (HE) 染色 (松岡俊樹)
脱パラフィン
↓ Xylen for 5min
↓ Xylen for 5min
↓ Xylen for 5min
↓ Xylen for 5min
親水
↓ 100% Ethanol for 3sec
↓ 100% Ethanol for 3sec
↓ 95% Ethanol for 3sec
↓ 90% Ethanol for 3sec
↓ 80% Ethanol for 3sec
↓ 流水洗浄(水道水) for 2~3min ←水をはじかなくなるまで行なう
(↓ PBS for 5min ×3 )
HE染色 (Mayer's Hematoxylin)
↓ Mayer's Hematoxylin液 for 15~20min
↓ 流水洗浄 ←洗浄水に色素の色がなくなるまで行なう
↓ 1% HCl・70% Ethanol for 1sec
↓ 流水洗浄
(↓ 飽和炭酸リチウム for 1sec) ←色出し
(↓ 流水洗浄(DW) for 10min)
↓ 蒸留水に軽く通す ←2~3回up-down
↓ eosin液 ←2~3回up-down
↓ 余分の液を流し去る程度の洗浄(DW)←2~3回up-down
脱水
↓ 70% Ethanol for 3sec
↓ 80% Ethanol for 3sec
↓ 90% Ethanol for 3sec
↓ 95% Ethanol for 3sec
↓ 100% Ethanol for 5sec
↓ 100% Ethanol for 5sec
透徹
↓ Xylen for 5min
↓ Xylen for 5min
↓ Xylen for 5min
↓ Xylen for 5min
封入
↓ EUKITT で封入